I think you mean the night club is single storey, not the building.
You could remove the door into the club from the stairs but this would not really be in keeping with ADB and you couldn't really claim that travel distance ends at the top step. But if you are risk assessing the issue then ADB is not a standard you have to use.
If you do want to allow the door to be removed you should treat the staircase as an accommodation stair (even though it is enclosed at ground floor) which means that no one should have to pass the head of the open staircase to reach the alternative staircase. Precisely, what "passing the head of the stairs" means is open to your interpretation but the 4.5m rule might be something to consider. If you don't treat it as an accommodation staircase then you run the risk of being very vulnerable to the risks that could be posed if a fire occurs downstairs and the single door into the ground floor lobby is not fully and effectively closed.
I'm assuming that the alternative exit is large enough to accommodate all occupants. I know it has to be, but I'm just saying.
I'm also assuming that there is no fire loading within the staircase you want to open up (not that this makes a huge amount of difference if you're treating the stairs as accommodation stairs).
Personally, my default position would be to advise against removal of the door at the head of the staircase because it weakens the overall fire safety measures present, but if the other elements of the risk assessment weigh in favour of a lightening of the installed fire safety provisions then I might accept the door's removal.